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蓝舌病病毒和流行性出血病病毒在BHK21细胞中的蚀斑中和作用

Plaque neutralization of bluetongue virus and epizootic hemorrhagic disease virus in BHK21 cells.

作者信息

Jochim M M, Jones S C

出版信息

Am J Vet Res. 1976 Nov;37(11):1345-7.

PMID:185929
Abstract

Plaque assay and plaque neutralization of blue-tongue virus and epizootic hemorrhagic disease virus were studied in baby hamster kidney (BHK21) cells grown under an overlay containing gum tragacanth. Tests were done in plastic panels, each with 24 wells, and variables were established for achieving reproducible results. Four serotypes of bluetongue virus were compared, and their antigenic differences were confirmed with this new plaque-neutralization test.

摘要

在含有黄芪胶的覆盖层下生长的幼仓鼠肾(BHK21)细胞中,研究了蓝舌病毒和流行性出血病病毒的蚀斑测定和蚀斑中和。试验在每个有24孔的塑料板中进行,并确定了变量以获得可重复的结果。比较了四种血清型的蓝舌病毒,并用这种新的蚀斑中和试验证实了它们的抗原差异。

相似文献

1
Plaque neutralization of bluetongue virus and epizootic hemorrhagic disease virus in BHK21 cells.蓝舌病病毒和流行性出血病病毒在BHK21细胞中的蚀斑中和作用
Am J Vet Res. 1976 Nov;37(11):1345-7.
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引用本文的文献

1
Simple procedure for preparation of bluetongue virus and epizootic hemorrhagic disease virus antigens for agar gel immunodiffusion.用于琼脂凝胶免疫扩散的蓝舌病病毒和流行性出血病病毒抗原的简易制备方法。
J Clin Microbiol. 1983 Dec;18(6):1310-3. doi: 10.1128/jcm.18.6.1310-1313.1983.
2
Tissue tropism and target cells of bluetongue virus in the chicken embryo.鸡胚中蓝舌病毒的组织嗜性和靶细胞
J Virol. 1988 Mar;62(3):887-93. doi: 10.1128/JVI.62.3.887-893.1988.
3
Epidemiology of bluetongue and related orbiviruses in the Sultanate of Oman.
阿曼苏丹国蓝舌病及相关环状病毒的流行病学
Epidemiol Infect. 1991 Feb;106(1):167-78. doi: 10.1017/s0950268800056533.
4
Comparisons of some bluetongue virus isolates by plaque neutralization and relatedness tests.通过蚀斑中和及相关性试验对一些蓝舌病毒分离株进行比较。
Arch Virol. 1979;62(3):189-99. doi: 10.1007/BF01317551.
5
A rapid plaque neutralization test for bluetongue virus.蓝舌病病毒快速蚀斑中和试验
Can J Comp Med. 1979 Apr;43(2):234-6.