Sugiyama M, Hirayama N, Sasaki H, Sugimura T, Minamoto N, Kinjo T
Department of Veterinary Public Health, Faculty of Agriculture, Gifu University, Japan.
Res Vet Sci. 1989 Mar;46(2):283-5.
Twelve monoclonal antibodies against ibaraki virus (IbV) were established and preliminarily characterised by indirect immunoperoxidase (IIP), haemagglutination inhibition (HI) and neutralisation (NT) tests. Five antibodies reacted in the IIP test with all IbV and epizootic haemorrhagic disease virus (EHDV) strains tested, and five antibodies reacted with IbV and Alberta strain (serotype 2) but not with New Jersey strain (serotype 1) of EHDV. Two of 12 antibodies showed both HI and NT activities. Viral proteins with molecular weights of about 24,000 daltons (24KD) and 78KD were determined by two monoclonal antibodies in Western blot analysis. One of two antibodies with the ability of both HI and NT recognised a viral protein with a molecular weight of about 78KD.
制备了12种抗茨城县病毒（IbV）的单克隆抗体，并通过间接免疫过氧化物酶（IIP）、血凝抑制（HI）和中和（NT）试验进行了初步表征。5种抗体在IIP试验中与所有测试的IbV和流行性出血病病毒（EHDV）毒株发生反应，5种抗体与IbV和艾伯塔毒株（血清型2）发生反应，但与EHDV的新泽西毒株（血清型1）不发生反应。12种抗体中有2种同时具有HI和NT活性。在蛋白质印迹分析中，两种单克隆抗体确定了分子量约为24000道尔顿（24KD）和78KD的病毒蛋白。具有HI和NT能力的两种抗体中的一种识别出一种分子量约为78KD的病毒蛋白。
