Bowen R A
Animal Reproduction Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
Am J Vet Res. 1987 Oct;48(10):1449-52.
Six calves were inoculated with 1 of 2 North American serotypes of epizootic hemorrhagic disease virus (EHDV) and then inoculated with the second serotype 16 weeks later. One calf did not develop an immune response to EHDV after primary inoculation and was removed from the study. Viremia after primary inoculation was transient. Although each infected calf developed a high serum neutralizing antibody titer to EHDV, at no time after inoculation with one or both viruses was antibody detected that neutralized any US serotypes of bluetongue virus. After exposure to both serotypes of EHDV, 4 of 5 calves developed antibodies that cross-reacted with group-specific bluetongue virus antigens.
六头小牛接种了两种北美血清型之一的流行性出血病病毒（EHDV），然后在16周后接种第二种血清型。一头小牛在初次接种后未对EHDV产生免疫反应，被排除在研究之外。初次接种后的病毒血症是短暂的。尽管每头受感染的小牛都对EHDV产生了高血清中和抗体滴度，但在接种一种或两种病毒后的任何时候，都未检测到中和任何美国血清型蓝舌病病毒的抗体。在接触两种血清型的EHDV后，5头小牛中有4头产生了与蓝舌病病毒群特异性抗原发生交叉反应的抗体。
