Deb Shirshendu K, Davis Brandon, Knudsen Giselle M, Gudihal Ravindra, Ben-Amotz Dor, Davisson V Jo
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA.
J Am Chem Soc. 2008 Jul 30;130(30):9624-5. doi: 10.1021/ja800772p. Epub 2008 Jul 1.
Accurate quantification of protein content and composition has been achieved using isotope-edited surface enhanced resonance Raman spectroscopy. Synthesis of isotopomeric Rhodamine dye-linked bioconjugation reagents enabled direct labeling of surface lysines on a variety of proteins. When separated in polyacrylamide gels and stained with silver nanoparticles. The spectral signatures reflect the expected statistical distribution of isotopomeric labels on the labeled proteins in the gel matrix format without interference from protein features.
使用同位素编辑的表面增强共振拉曼光谱法已实现对蛋白质含量和组成的准确量化。合成同位素异构体罗丹明染料连接的生物共轭试剂能够直接标记多种蛋白质上的表面赖氨酸。当在聚丙烯酰胺凝胶中分离并用银纳米颗粒染色时，光谱特征反映了凝胶基质形式的标记蛋白质上同位素异构体标记的预期统计分布，而不受蛋白质特征的干扰。
