Peng Bo, Qin Yu
Department of Chemistry, Renmin University of China, Beijing, China, 100872.
Anal Chem. 2008 Aug 1;80(15):6137-41. doi: 10.1021/ac800946p. Epub 2008 Jul 2.
An optical sensing approach for the detection of saccharides based on reversible boronic acid-diol complexation in a lipophilic polymer membrane is presented. The complexation of saccharides with phenyl boronic acids that are immobilized in a hydrophobic polymer matrix produces a stable boronate anion and liberates a mobile hydrogen ion. The change in fluorescence intensity of the sensing films resulting from the increase in hydrogen ion concentration is thus directly related to the ambient saccharide concentration. By monitoring the pH change in a bulk optical film, we were able to detect D-glucose, D-fructose, D-galactose, and D-sorbitol with a concentration range from 0.1 to 100 mM at physiological pH 7.4.
本文提出了一种基于亲脂性聚合物膜中可逆硼酸 - 二醇络合作用检测糖类的光学传感方法。糖类与固定在疏水聚合物基质中的苯基硼酸络合会产生稳定的硼酸根阴离子并释放出可移动的氢离子。因此，氢离子浓度增加导致传感膜荧光强度的变化与周围糖类浓度直接相关。通过监测整体光学膜中的pH变化，我们能够在生理pH值7.4下检测浓度范围为0.1至100 mM的D - 葡萄糖、D - 果糖、D - 半乳糖和D - 山梨醇。
