Rötting Anna K, Freeman David E, Constable Peter D, Eurell Jo Ann C, Wallig Matthew A
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.
Am J Vet Res. 2008 Jul;69(7):874-9. doi: 10.2460/ajvr.69.7.874.
To establish reference values for the range of the number of eosinophils found in equine gastrointestinal mucosa and to describe the distribution of this cell within the equine gastrointestinal mucosa.
Gastrointestinal mucosal specimens from 14 adult horses euthanatized for reasons other than gastrointestinal disease.
Gastrointestinal mucosal specimens were collected and grouped according to their anatomic regions. For histologic examination slides were stained with Luna's eosinophil stain to determine eosinophil accumulation and distribution. The mucosa was divided into 5 sections for each anatomic location, and the percentage of eosinophils in each of the 5 sections relative to the total eosinophil count in all sections was determined. Additionally, the number of eosinophils per square millimeter of mucosa was calculated as a measure of the degree of eosinophil accumulation.
Lowest numbers of eosinophils were found in the stomach, and numbers increased from there to the cecum, then decreased from the ascending colon (right ventral colon, left ventral colon, pelvic flexure, left dorsal colon, and right dorsal colon) to small colon. In all gastrointestinal sections, most eosinophils were located near the muscularis mucosae and were rarely found near or on the luminal surface of the mucosa.
The distribution of eosinophils in the gastrointestinal tract of horses followed a pattern within the mucosa and between different sections of the gastrointestinal tract. The derived reference values and distribution data could be used to detect changes in eosinophil response in the equine gastrointestinal mucosa caused by diseases states.
确定马属动物胃肠道黏膜中嗜酸性粒细胞数量范围的参考值，并描述该细胞在马属动物胃肠道黏膜内的分布情况。
14匹因非胃肠道疾病原因实施安乐死的成年马的胃肠道黏膜标本。
收集胃肠道黏膜标本，并根据其解剖区域进行分组。为进行组织学检查，玻片用卢娜嗜酸性粒细胞染色剂染色，以确定嗜酸性粒细胞的聚集和分布情况。每个解剖部位的黏膜被分为5个部分，确定这5个部分中嗜酸性粒细胞数量相对于所有部分嗜酸性粒细胞总数的百分比。此外，计算每平方毫米黏膜中嗜酸性粒细胞的数量，作为嗜酸性粒细胞聚集程度的指标。
胃中嗜酸性粒细胞数量最少，从胃到盲肠数量增加，然后从升结肠（右腹侧结肠、左腹侧结肠、骨盆弯曲部、左背侧结肠和右背侧结肠）到小结肠数量减少。在所有胃肠道部位，大多数嗜酸性粒细胞位于黏膜肌层附近，在黏膜腔面附近或表面很少发现。
马属动物胃肠道中嗜酸性粒细胞的分布在黏膜内以及胃肠道不同部位之间遵循一种模式。得出的参考值和分布数据可用于检测疾病状态引起的马属动物胃肠道黏膜嗜酸性粒细胞反应的变化。
