脂肪来源干细胞：分离、扩增与分化
Adipose-derived stem cells: isolation, expansion and differentiation.
作者信息
Bunnell Bruce A, Flaat Mette, Gagliardi Christine, Patel Bindiya, Ripoll Cynthia
机构信息
Division of Gene Therapy, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433, USA.
出版信息
Methods. 2008 Jun;45(2):115-20. doi: 10.1016/j.ymeth.2008.03.006. Epub 2008 May 29.
Abstract
The emerging field of regenerative medicine will require a reliable source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose tissue has proven to serve as an abundant, accessible and rich source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. There has been increased interest in adipose-derived stem cells (ASCs) for tissue engineering applications. Here, methods for the isolation, expansion and differentiation of ASCs are presented and described in detail. While this article has focused on the isolation of ASCs from human adipose tissue, the procedure can be applied to adipose tissues from other species with minimal modifications.
摘要
除了生物材料支架和细胞因子生长因子外，再生医学这一新兴领域还需要可靠的干细胞来源。脂肪组织已被证明是成体干细胞的丰富、易获取且丰富的来源，具有适合组织工程和再生医学应用的多能特性。人们对用于组织工程应用的脂肪来源干细胞（ASC）的兴趣日益增加。本文详细介绍并描述了ASC的分离、扩增和分化方法。虽然本文重点关注从人脂肪组织中分离ASC，但该程序只需进行最少的修改即可应用于其他物种的脂肪组织。
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