Bosman Lisa Marie, Ambele Melvin A, Pepper Michael S
Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Methods Mol Biol. 2025;2938:171-185. doi: 10.1007/978-1-0716-4607-6_19.
Regenerative medicine is a relatively new branch of therapeutics in equine medicine, which aims to restore and reconstitute tissue function and structure via cellular and/or noncellular approaches. Biological constituents such as mesenchymal stromal/stem cells (MSCs) are potent therapeutics, which can aid in damaged tissue regeneration due to their differentiation capacity into many different cell types such as adipose tissue, bone, and cartilage. MSCs can be successfully and conveniently isolated from equine subcutaneous adipose tissue (adipose-derived stromal cells, ASCs). In horses, there are currently no standardized methods for characterizing ASCs both in research and clinical practice. However, many studies are investigating antibodies which may be expressed on the ASC cell surface through multicolor immunophenotyping. This chapter describes techniques used to isolate, grow, characterize, and store equine ASCs, which have been adapted from extensive published research from the Institute of Cellular and Molecular Medicine (ICMM) at the University of Pretoria.
再生医学是马医学中一个相对较新的治疗分支，旨在通过细胞和/或非细胞方法恢复和重建组织功能与结构。间充质基质/干细胞（MSCs）等生物成分是有效的治疗手段，因其能够分化为多种不同类型的细胞，如脂肪组织、骨骼和软骨，从而有助于受损组织的再生。MSCs可以从马的皮下脂肪组织（脂肪来源的基质细胞，ASCs）中成功且便捷地分离出来。在马的研究和临床实践中，目前尚无标准化的方法来鉴定ASCs。然而，许多研究正在探究通过多色免疫表型分析可能在ASC细胞表面表达的抗体。本章描述了用于分离、培养、鉴定和储存马ASCs的技术，这些技术改编自比勒陀利亚大学细胞与分子医学研究所（ICMM）广泛发表的研究成果。
