我该留下还是离开？真核生物翻译起始因子1和1A控制起始密码子识别。
Should I stay or should I go? Eukaryotic translation initiation factors 1 and 1A control start codon recognition.
作者信息
Mitchell Sarah F, Lorsch Jon R
机构信息
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
出版信息
J Biol Chem. 2008 Oct 10;283(41):27345-27349. doi: 10.1074/jbc.R800031200. Epub 2008 Jun 30.
Abstract
Start codon selection is a key step in translation initiation as it sets the reading frame for decoding. Two eukaryotic initiation factors, eIF1 and eIF1A, are key actors in this process. Recent work has elucidated many details of the mechanisms these factors use to control start site selection. eIF1 prevents the irreversible GTP hydrolysis that commits the ribosome to initiation at a particular codon. eIF1A both promotes and inhibits commitment through the competing influences of its two unstructured termini. Both factors perform their tasks through a variety of interactions with other components of the initiation machinery, in many cases mediated by the unstructured regions of the two proteins.
摘要
起始密码子的选择是翻译起始过程中的关键步骤，因为它为解码设定了阅读框。两个真核生物起始因子，即eIF1和eIF1A，是这一过程中的关键角色。最近的研究阐明了这些因子用于控制起始位点选择的机制的许多细节。eIF1可防止核糖体在特定密码子处启动时发生不可逆的GTP水解。eIF1A通过其两个无结构末端的竞争性影响，既促进又抑制起始。这两个因子都通过与起始机制的其他组分的多种相互作用来执行它们的任务，在许多情况下是由这两种蛋白质的无结构区域介导的。
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