Levine L
Department of Biochemistry, Brandeis University, Waltham, MA 02254.
Biochem Biophys Res Commun. 1991 Jul 31;178(2):641-7. doi: 10.1016/0006-291x(91)90156-2.
Preincubation of rat liver cells (the C-9 cell line) for 25 min with phenylarsine oxide at levels ranging from 0.06 to 0.6 microM amplifies prostaglandin I2 production when subsequently stimulated by platelet activating factor, lysine vasopressin, bradykinin, thapsigargin, and the Ca2+ ionophore, A-23187, but not that stimulated by exogenous arachidonic acid. The amplification is decreased after preincubation for 25 min with 1.8 microM phenylarsine oxide. Preincubation of mouse lymphoma cells (the WEHI-3 cell line) with phenylarsine oxide at levels ranging from 0.06 to 1.8 microM for 60 min does not affect prostaglandin E2 levels but inhibits leukotriene B4 and C4 production stimulated by the Ca(2+)-ionophore, A-23187. Amplification of prostaglandin production by phenylarsine oxide is reversed 100 times more effectively by 2,3-dimercaptopropanol than by 2-mercaptoethanol. Deesterification of lipids appears to be regulated positively in rat liver cells and leukotriene production negatively in mouse lymphoma cells by phosphorylation of tyrosine.
将大鼠肝细胞（C - 9细胞系）与浓度范围为0.06至0.6微摩尔的苯胂氧化物预孵育25分钟，随后受到血小板活化因子、赖氨酸加压素、缓激肽、毒胡萝卜素和Ca2 +离子载体A - 23187刺激时，会增强前列腺素I2的生成，但受到外源性花生四烯酸刺激时则不然。用1.8微摩尔苯胂氧化物预孵育25分钟后，这种增强作用会减弱。将小鼠淋巴瘤细胞（WEHI - 3细胞系）与浓度范围为0.06至1.8微摩尔的苯胂氧化物预孵育60分钟，不会影响前列腺素E2水平，但会抑制Ca(2 +)离子载体A - 23187刺激的白三烯B4和C4的生成。苯胂氧化物对前列腺素生成的增强作用，2,3 - 二巯基丙醇的逆转效果比2 - 巯基乙醇有效100倍。脂质的去酯化作用在大鼠肝细胞中似乎受到酪氨酸磷酸化的正向调节，而在小鼠淋巴瘤细胞中白三烯的生成则受到负向调节。
