Levine L
Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254.
Prostaglandins. 1991 Jan;41(1):7-19. doi: 10.1016/0090-6980(91)90100-t.
Sodium vanadate (11 microM) amplified the PGI2 production of rat liver cells (the C-9 cell line) incubated with thrombin, platelet activating factor, lysine-vasopressin, the Ca2(+)-ionophore A-23187, interleukin-1 beta, 12-tetradecanoylphorbol-13-acetate, teleocidin, epidermal growth factor, palytoxin, thapsigargin and colchicine but not that stimulated by exogenous arachidonic acid. Sodium vanadate (2.2 microM) also amplified PGF2 alpha production of dog kidney cells (the MDCK cell line) incubated with norepinephrine and, at 0.4 microM, PGI2 production of bovine aorta smooth muscle cells stimulated by serotonin. Sodium vanadate (55 microM) did not affect production of PGE2 and PGF2 alpha in rat basophil leukemia cells (the RBL-1 cell line) stimulated by the Ca2(+)-ionophore A-23187, but did inhibit synthesis of peptide-containing leukotrienes and 12-hydroxyeicosatetraenoic acid. When used with cultured cells at micromolar concentrations, vanadate is known to inhibit protein tyrosine-phosphate phosphatases. These results suggest that in some cells deesterification of lipids is positively regulated, at least in part, by phosphorylation of tyrosine whereas in leukocytes, lipoxygenase activities are negatively regulated, at least in part, by phosphorylation of tyrosine.
钒酸钠（11微摩尔）可增强经凝血酶、血小板活化因子、赖氨酸加压素、钙离子载体A-23187、白细胞介素-1β、12-十四烷酰佛波醇-13-乙酸酯、远侧霉素、表皮生长因子、岩沙海葵毒素、毒胡萝卜素和秋水仙碱处理的大鼠肝细胞（C-9细胞系）的前列环素（PGI2）生成，但不影响外源性花生四烯酸刺激的PGI2生成。钒酸钠（2.2微摩尔）还可增强经去甲肾上腺素处理的犬肾细胞（MDCK细胞系）的前列腺素F2α（PGF2α）生成，以及在0.4微摩尔浓度下血清素刺激的牛主动脉平滑肌细胞的PGI2生成。钒酸钠（55微摩尔）不影响钙离子载体A-23187刺激的大鼠嗜碱性白血病细胞（RBL-1细胞系）中前列腺素E2（PGE2）和PGF2α的生成，但可抑制含肽白三烯和12-羟基二十碳四烯酸的合成。已知当以微摩尔浓度与培养细胞一起使用时，钒酸盐可抑制蛋白质酪氨酸磷酸酶。这些结果表明，在某些细胞中，脂质的去酯化至少部分地受到酪氨酸磷酸化的正调控，而在白细胞中，脂氧合酶活性至少部分地受到酪氨酸磷酸化的负调控。
