Garson L, Gray V
Department of Botany, University of the Witwatersrand, Johannesburg, South Africa.
Biochem Int. 1991 Jan;23(2):299-305.
Nitrate and alanine were found to stimulate partially purified maize leaf phosphoenolpyruvate carboxylase under specific assay conditions. Both metabolites stimulated the enzyme at low pH (7.0-7.5) and low substrate levels (1mM phosphoenolpyruvate). Nitrate was found to have a biphasic effect on the enzyme, stimulating at low concentrations (1mM-3mM), with a decrease in stimulation at higher levels. Nitrate caused inhibition of activity at pH 8.0 and although alanine caused some stimulation in activity at pH 8.0 this was not as marked as at the lower pH levels.
在特定的测定条件下，发现硝酸盐和丙氨酸能刺激部分纯化的玉米叶片磷酸烯醇式丙酮酸羧化酶。在低pH值（7.0 - 7.5）和低底物水平（1 mM磷酸烯醇式丙酮酸）时，这两种代谢物都能刺激该酶。发现硝酸盐对该酶有双相作用，在低浓度（1 mM - 3 mM）时起刺激作用，在较高浓度时刺激作用减弱。在pH 8.0时硝酸盐会抑制活性，尽管丙氨酸在pH 8.0时能引起一定程度的活性刺激，但不如在较低pH值时明显。
