Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota 55455.
Biotechnol Bioeng. 1990 Jun 20;36(2):191-7. doi: 10.1002/bit.260360211.
Measurements of volume distributions and dry weight are made on hybridoma cells in culture. The volume of viable hybridoma cells is significantly larger than that of nonviable cells. During exponential growth, the volume of the viable hybridoma cells is found to be significantly larger than that during other stages of batch culture. Proportionality is found between the volume of the cells and their dry weight, indicating that the volume data can be used in conjunction with cell concentration data as a practical technique for indirect measurement of the biomass concentration present in a culture. Comparison of dry weight concentrations in continuous culture to predictions from the volume data shows very good agreement.
对培养中的杂交瘤细胞进行体积分布和干重测量。活杂交瘤细胞的体积明显大于非活细胞。在指数生长期，活杂交瘤细胞的体积明显大于批培养的其他阶段。细胞体积与其干重之间存在比例关系，表明可以将体积数据与细胞浓度数据结合起来，作为间接测量培养物中生物质浓度的实用技术。将连续培养中的干重浓度与体积数据的预测值进行比较，结果非常吻合。
