人偏肺病毒F蛋白介导的低pH诱导膜融合是一种罕见的、毒株依赖性现象。
Low-pH-induced membrane fusion mediated by human metapneumovirus F protein is a rare, strain-dependent phenomenon.
作者信息
Herfst Sander, Mas Vicente, Ver Lorena S, Wierda Rutger J, Osterhaus Albert D M E, Fouchier Ron A M, Melero José A
机构信息
Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands.
出版信息
J Virol. 2008 Sep;82(17):8891-5. doi: 10.1128/JVI.00472-08. Epub 2008 Jul 2.
Abstract
Membrane fusion promoted by human metapneumovirus (HMPV) fusion (F) protein was suggested to require low pH (R. M. Schowalter, S. E. Smith, and R. E. Dutch, J. Virol. 80:10931-10941, 2006). Using prototype F proteins representing the four HMPV genetic lineages, we detected low-pH-dependent fusion only with some lineage A proteins and not with lineage B proteins. A glycine at position 294 was found responsible for the low-pH requirement in lineage A proteins. Only 6% of all HMPV lineage A F sequences have 294G, and none of the lineage B sequences have 294G. Thus, acidic pH is not a general trigger of HMPV F proteins for activity.
摘要
人偏肺病毒（HMPV）融合（F）蛋白促进的膜融合被认为需要低pH值（R. M. 肖沃尔特、S. E. 史密斯和R. E. 达奇，《病毒学杂志》80:10931 - 10941，2006年）。使用代表HMPV四个遗传谱系的原型F蛋白，我们仅在某些A谱系蛋白中检测到低pH依赖性融合，而在B谱系蛋白中未检测到。发现A谱系蛋白中第294位的甘氨酸是低pH要求的原因。所有HMPV A谱系F序列中只有6%具有294G，而B谱系序列中没有一个具有294G。因此，酸性pH不是HMPV F蛋白激活的普遍触发因素。
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