突变型单纯疱疹病毒1型DNA聚合酶增强的DNA复制保真度是由提高的核苷酸选择性和降低的错配延伸能力介导的。
The enhanced DNA replication fidelity of a mutant herpes simplex virus type 1 DNA polymerase is mediated by an improved nucleotide selectivity and reduced mismatch extension ability.
作者信息
Tian Wang, Hwang Ying T, Hwang Charles B C
机构信息
Department of Microbiology and Immunology, State University of New York, Upstate Medical University, Syracuse, New York 13210, USA.
出版信息
J Virol. 2008 Sep;82(17):8937-41. doi: 10.1128/JVI.00911-08. Epub 2008 Jul 2.
Abstract
We previously demonstrated that a recombinant herpes simplex virus containing a mutation within the finger domain of DNA polymerase replicated DNA with increased fidelity. In this study, we demonstrate that, compared with wild-type polymerase, the mutant enzyme exhibited improved nucleotide selectivity and a reduced ability to extend from mismatched primer termini, which would contribute to the increased DNA replication fidelity.
摘要
我们之前证明，一种在DNA聚合酶指状结构域内含有突变的重组单纯疱疹病毒能够以更高的保真度复制DNA。在本研究中，我们证明，与野生型聚合酶相比，突变酶表现出改善的核苷酸选择性以及从错配引物末端延伸的能力降低，这将有助于提高DNA复制保真度。
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