有证据表明，与单纯疱疹病毒1型DNA聚合酶相关的核酸酶活性是由3'-5'外切核酸酶引起的。
Evidence that the nuclease activities associated with the herpes simplex type 1 DNA polymerase are due to the 3'-5' exonuclease.
作者信息
Hall J D, Orth K L, Claus-Walker D
机构信息
Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA.
出版信息
J Virol. 1996 Jul;70(7):4816-8. doi: 10.1128/JVI.70.7.4816-4818.1996.
Abstract
We investigated nuclease activities associated with the catalytic subunit of herpes simplex virus type 1 DNA polymerase. We confirm that a 3'-5' exonuclease copurifies with this enzyme. Previous reports suggested that a 5' DNase was intrinsic to the polymerase. Our preparation lacks such activity.
摘要
我们研究了与单纯疱疹病毒1型DNA聚合酶催化亚基相关的核酸酶活性。我们证实一种3'-5'核酸外切酶与该酶共纯化。先前的报道表明该聚合酶固有5'脱氧核糖核酸酶活性。而我们的制备物缺乏这种活性。
相似文献
1
Evidence that the nuclease activities associated with the herpes simplex type 1 DNA polymerase are due to the 3'-5' exonuclease.
J Virol. 1996 Jul;70(7):4816-8. doi: 10.1128/JVI.70.7.4816-4818.1996.
2
Herpes simplex virus type 1 DNA polymerase. Mutational analysis of the 3'-5'-exonuclease domain.
J Biol Chem. 1996 Nov 15;271(46):29245-54. doi: 10.1074/jbc.271.46.29245.
3
Impaired mismatch extension by a herpes simplex DNA polymerase mutant with an editing nuclease defect.
J Biol Chem. 1998 Sep 11;273(37):24075-82. doi: 10.1074/jbc.273.37.24075.
4
The enhanced DNA replication fidelity of a mutant herpes simplex virus type 1 DNA polymerase is mediated by an improved nucleotide selectivity and reduced mismatch extension ability.
J Virol. 2008 Sep;82(17):8937-41. doi: 10.1128/JVI.00911-08. Epub 2008 Jul 2.
5
Mutations within conserved motifs in the 3'-5' exonuclease domain of herpes simplex virus DNA polymerase.
J Gen Virol. 1995 Dec;76 ( Pt 12):2999-3008. doi: 10.1099/0022-1317-76-12-2999.
6
Finger domain mutation affects enzyme activity, DNA replication efficiency, and fidelity of an exonuclease-deficient DNA polymerase of herpes simplex virus type 1.
J Virol. 2009 Jul;83(14):7194-201. doi: 10.1128/JVI.00632-09. Epub 2009 May 6.
7
Herpes Simplex Virus 1 DNA Polymerase RNase H Activity Acts in a 3'-to-5' Direction and Is Dependent on the 3'-to-5' Exonuclease Active Site.
J Virol. 2018 Feb 12;92(5). doi: 10.1128/JVI.01813-17. Print 2018 Mar 1.
8
HSV-1 DNA polymerase 3'-5' exonuclease-deficient mutant D368A exhibits severely reduced viral DNA synthesis and polymerase expression.
J Gen Virol. 2018 Oct;99(10):1432-1437. doi: 10.1099/jgv.0.001138. Epub 2018 Sep 3.
9
Herpes simplex-1 DNA polymerase. Identification of an intrinsic 5'----3' exonuclease with ribonuclease H activity.
J Biol Chem. 1989 Nov 15;264(32):19266-70.
10
Effects of Acyclovir, Foscarnet, and Ribonucleotides on Herpes Simplex Virus-1 DNA Polymerase: Mechanistic Insights and a Novel Mechanism for Preventing Stable Incorporation of Ribonucleotides into DNA.
Biochemistry. 2016 Feb 23;55(7):1168-77. doi: 10.1021/acs.biochem.6b00065. Epub 2016 Feb 11.
引用本文的文献
1
Herpes Simplex Virus 1 DNA Polymerase RNase H Activity Acts in a 3'-to-5' Direction and Is Dependent on the 3'-to-5' Exonuclease Active Site.
J Virol. 2018 Feb 12;92(5). doi: 10.1128/JVI.01813-17. Print 2018 Mar 1.
2
Herpes simplex virus processivity factor UL42 imparts increased DNA-binding specificity to the viral DNA polymerase and decreased dissociation from primer-template without reducing the elongation rate.
J Virol. 1999 Jan;73(1):55-66. doi: 10.1128/JVI.73.1.55-66.1999.
3
Evolution of viral DNA-dependent DNA polymerases.
Virus Genes. 1998;16(1):47-58. doi: 10.1023/a:1007997609122.
本文引用的文献
1
Mutations within conserved motifs in the 3'-5' exonuclease domain of herpes simplex virus DNA polymerase.
J Gen Virol. 1995 Dec;76 ( Pt 12):2999-3008. doi: 10.1099/0022-1317-76-12-2999.
2
Structural and functional organization of herpes simplex virus DNA polymerase investigated by limited proteolysis.
J Biol Chem. 1994 Sep 9;269(36):22788-96.
3
Herpes simplex-1 DNA polymerase. Identification of an intrinsic 5'----3' exonuclease with ribonuclease H activity.
J Biol Chem. 1989 Nov 15;264(32):19266-70.
4
The herpes simplex virus type I DNA polymerase. Polypeptide structure and antigenic domains.
Eur J Biochem. 1988 Jul 1;174(4):707-16. doi: 10.1111/j.1432-1033.1988.tb14155.x.
5
The herpes simplex virus type 1 UL42 gene product: a subunit of DNA polymerase that functions to increase processivity.
J Virol. 1990 Dec;64(12):5976-87. doi: 10.1128/JVI.64.12.5976-5987.1990.
6
Comparison of exonucleolytic activities of herpes simplex virus type-1 DNA polymerase and DNase.
Eur J Biochem. 1990 Jul 31;191(2):263-73. doi: 10.1111/j.1432-1033.1990.tb19119.x.
7
Enzymatic activities of overexpressed herpes simplex virus DNA polymerase purified from recombinant baculovirus-infected insect cells.
Nucleic Acids Res. 1990 Mar 11;18(5):1207-15. doi: 10.1093/nar/18.5.1207.
8
A general structure for DNA-dependent DNA polymerases.
Gene. 1991 Apr;100:27-38. doi: 10.1016/0378-1119(91)90346-d.
9
Properties of herpes simplex virus DNA polymerase and characterization of its associated exonuclease activity.
Eur J Biochem. 1979 Jul;98(1):231-44. doi: 10.1111/j.1432-1033.1979.tb13181.x.
Zotero 插件