Connally Russell E, Piper James A
Centre for Laser Applications, Department of Physics, Division of Information and Communication Sciences, Macquarie University, Sydney, NSW 2109, Australia.
Ann N Y Acad Sci. 2008;1130:106-16. doi: 10.1196/annals.1430.032.
Autofluorescent algal samples were spiked with europium beads for analysis on a novel all-solid-state, time-gated luminescence (TGL) microscope. Pulsed UV excitation (365 nm) was provided by a high-power UV-LED source fitted to an Olympus BX51 microscope. An "Impactron" electron multiplying charge-coupled-device (CCD) camera acquired images in delayed luminescence mode. Second, we evaluated sensitivity of the instrument by acquiring images of immunofluorescently labeled Giardia cysts with a single-exposure period of 3 ms. The camera was triggered 3 micros after the LED had extinguished to yield a 14-fold increase in signal-to-noise ratio within a single 33 ms capture cycle. This novel instrument could be switched instantly from prompt epifluorescence mode to TGL mode for suppression of short-lived fluorescence.
将铕珠添加到自发荧光藻类样本中，以便在新型全固态时间分辨荧光（TGL）显微镜上进行分析。脉冲紫外激发（365nm）由安装在奥林巴斯BX51显微镜上的高功率紫外发光二极管光源提供。一台“Impactron”电子倍增电荷耦合器件（CCD）相机以延迟发光模式采集图像。其次，我们通过在3毫秒的单曝光时间内采集免疫荧光标记的贾第虫囊肿图像来评估该仪器的灵敏度。在发光二极管熄灭3微秒后触发相机，以便在单个33毫秒的捕获周期内使信噪比提高14倍。这种新型仪器可以立即从即时落射荧光模式切换到TGL模式，以抑制短寿命荧光。
