Macquarie University, Balaclava Road, North Ryde Sydney, NSW, 2109, Australia.
Anal Chem. 2011 Jun 15;83(12):4782-7. doi: 10.1021/ac200281h. Epub 2011 May 19.
The sensitive detection of delayed luminescence (τ > 100 μs) can be achieved with an optomechanical instrument, the gated autosynchronous luminescence detector (GALD). The device effectively combines an excitation chopper, a detection chopper, and a dichroic mirror into a single element. With dimensions of 12 mm (H), 32 mm (W), and 90 mm (L), it is designed for insertion into the differential interference contrast (DIC) prism slot of a BX51 Olympus microscope. The GALD described here employed a compact high-power UV LED as the excitation source to capture images of Giardia lamblia cysts indirectly labeled with a europium chelate/streptavidin conjugate. Labeled cells were clearly visible in the complete absence of autofluorescence and signal intensity was sufficient to capture high-resolution color images within several seconds. Shorter exposure intervals of 100 ms on a monochrome Andor iXON camera delivered time-gated luminescence images with a signal-to-noise ratio better than 114:1.
利用光机械仪器——门控自动同步发光探测器（GALD），可以实现对延迟发光（τ>100 μs）的灵敏检测。该设备有效地将激发斩波器、检测斩波器和二向色镜组合成一个单一元件。其尺寸为 12mm（高）、32mm（宽）和 90mm（长），设计用于插入 Olympus 显微镜的微分干涉对比（DIC）棱镜槽中。这里描述的 GALD 采用紧凑型高功率 UV LED 作为激发源，间接捕获用铕螯合物/链霉亲和素缀合物标记的蓝氏贾第鞭毛虫包囊的图像。在完全没有自发荧光的情况下，标记的细胞清晰可见，信号强度足以在几秒钟内捕获高分辨率彩色图像。单色 Andor iXON 相机的曝光间隔为 100 ms 时，可以获得信噪比优于 114:1 的时间门控发光图像。
