Jung Gregor, Schmitt Alexander, Jacob Michaela, Hinkeldey Babette
Saarland University, Biophysical Chemistry, Campus, Building B2 2, Saarbruecken, Germany.
Ann N Y Acad Sci. 2008;1130:131-7. doi: 10.1196/annals.1430.006.
We highlight our recent achievements in the design of fluorescent dyes for the investigation of chemical reactions on the single-molecule level. These fluorophores are tailored to undergo changes in their photophysical properties upon chemical transformations. Three examples are presented: electrophilic aromatic substitution, phosphoester cleavage, and oxidation of double-bonds. Thin-layer chromatography and fluorescence correlation spectroscopy are used to separate and characterize the different reaction products, respectively. We are planning to develop more fluorescent synthons which enable us to perform single-molecule chemistry of various reactions.
我们重点介绍了我们近期在设计用于单分子水平化学反应研究的荧光染料方面所取得的成就。这些荧光团经过定制，可在化学转化时其光物理性质发生变化。文中给出了三个例子：亲电芳香取代、磷酸酯裂解和双键氧化。分别使用薄层色谱法和荧光相关光谱法来分离和表征不同的反应产物。我们正计划开发更多的荧光合成子，使我们能够进行各种反应的单分子化学研究。
