Hänninen Pekka, Soukka Jori, Soini Juhani T
Department of Cell Biology and Anatomy, Laboratory of Biophysics, University of Turku, 20014 Turku, Finland.
Ann N Y Acad Sci. 2008;1130:320-6. doi: 10.1196/annals.1430.040.
Application of two-photon excitation of fluorescence in microscopy is one of the major discoveries of the "renaissance" of light microscopy that started in the 1980s. The technique derives its advantages from the biologically "smooth" wavelength of the excitation light and the confinement of the excitation. Difficult, and seemingly nontransparent, samples may be imaged with the technique with good resolution. Although the bioresearch has been concentrating mostly on the positive properties of the technique for imaging, the same properties may be applied successfully to nonimaging bioassays. This article focuses on the development path of two-photon excitation-based assay system.
荧光双光子激发在显微镜中的应用是始于20世纪80年代的光学显微镜“复兴”的重大发现之一。该技术的优势源于激发光具有生物学上的“平滑”波长以及激发的局限性。对于难以处理且看似不透明的样本，该技术可以实现高分辨率成像。尽管生物研究大多集中在该技术用于成像的积极特性上，但这些特性也可以成功应用于非成像生物检测。本文重点介绍基于双光子激发的检测系统的发展历程。
