Maurer J, Mann W, Heinrich U R
ENT Department, University of Mainz, Federal Republic of Germany.
Eur Arch Otorhinolaryngol. 1991;248(4):242-5. doi: 10.1007/BF00173664.
Five guinea pigs were exposed to an interrupted 90 dB SPL pure tone of 3.2 kHz for a total application time of 5 min. Following sound application all animals were decapitated and the cochleae were removed. After that, calcium-binding sites were located by the potassium pyroantimonate precipitation method. Another three animals served as control animals and did not receive the sound treatment. Findings confirmed previous studies showing the spatial arrangements of precipitate rich regions in the inner ear's two acellular structures (the basilar membrane and tectorial membrane) and the two cellular structures (the inner hair cells and Huschke's teeth). By using semiquantitative evaluation we found a relative diminution of precipitable calcium in inner hair cells and in Huschke's teeth of sound-exposed animals when compared to untreated animals.
将五只豚鼠暴露于3.2kHz、90dB SPL的间断纯音下，总作用时间为5分钟。声音作用后，所有动物均被断头并取出耳蜗。之后，通过焦锑酸钾沉淀法定位钙结合位点。另外三只动物作为对照动物，未接受声音处理。研究结果证实了先前的研究，显示了内耳两个无细胞结构（基底膜和盖膜）以及两个细胞结构（内毛细胞和胡施克齿）中富含沉淀物区域的空间排列。通过半定量评估，我们发现与未处理动物相比，声音暴露动物的内毛细胞和胡施克齿中可沉淀钙相对减少。
