Hochkoeppler A, Luisi P L
Istituto Colture Industriali, 40129 Bologna, Italy.
Biotechnol Bioeng. 1991 Apr 25;37(10):918-21. doi: 10.1002/bit.260371004.
With the aim of possibly extending plant microbiology and photosynthesis beyond the usual applicability in aqueous solution, we investigated the solubilization of plant cells inorganic media with the help of water-in-oil microemulsions. Cells isolated from leaves of Rumex obtusifolius were solubilized in a water/2-ethyl-hexyl-sodiumsulfosuccinate/isooctane system, containing 20% water (v:v) and 240 mM surfactant, and the oxygen evolution/consumption was measured polarographically. Although no oxygen evolution was detectable in the organic medium, the cells were able to carry out photosynthetic oxygen consumption at the expense of ascorbate. To a lesser extent, photosynthetic oxygen consumption was measured using N, N, N', N'-tetramethyl-p-phenylenediamine as electron donor. The rate of ascorbate photooxidation was linearly related to the concentration of cells.
为了有可能将植物微生物学和光合作用扩展到超出其在水溶液中的通常适用范围，我们借助油包水微乳液研究了植物细胞在无机介质中的增溶作用。从钝叶酸模叶片中分离出的细胞在含有20%水（体积比）和240 mM表面活性剂的水/二（2-乙基己基）磺基琥珀酸钠/异辛烷体系中增溶，并通过极谱法测量氧气的释放/消耗。尽管在有机介质中未检测到氧气释放，但细胞能够以抗坏血酸为代价进行光合耗氧。在较小程度上，使用N，N，N'，N'-四甲基对苯二胺作为电子供体测量了光合耗氧。抗坏血酸光氧化速率与细胞浓度呈线性相关。
