Institut für Polymere, ETH-Zürich, Universitätserasse 6, 8092 Zürich, Switzerland.
Biotechnol Bioeng. 1992 Jun 5;40(1):173-8. doi: 10.1002/bit.260400124.
The solubilization and the photosynthetic activity of cyanobacteria (Anabaena variabilis) in water-in-oil microemulsions consisting of (Tween85/Span80)/hexadecane/water is investigated. Transparent and stable solutions containing up to 10(8) cells/mL could be obtained. The physical state and stability of the cells in the microemulsion, as evidenced from optical and electron microscopy, is dependent upon the physical parameters of the system, and in particular on the hydrophilic-lypophilic balance (HLB) of the surfactant system. Conditions could be found, under which the cells in the microemulsion system display photosynthetic activity This was judged by measuring polarographically the oxygen evolution and by studying the photosynthetic activity in the presence of specific inhibitors.
研究了由（Tween85/Span80）/十六烷/水组成的油包水乳状液中，蓝藻（鱼腥藻）的增溶和光合作用活性。可以获得高达 10(8)个细胞/mL 的透明且稳定的溶液。从光镜和电子显微镜可以看出，细胞在微乳液中的物理状态和稳定性取决于体系的物理参数，特别是取决于表面活性剂体系的亲水亲油平衡（HLB）。可以找到在微乳液体系中，细胞显示光合作用活性的条件，这是通过测定氧的光极法释放和在存在特定抑制剂的情况下研究光合作用活性来判断的。
