枯草芽孢杆菌RecO将RecA聚集到单链结合蛋白A（SsbA）包被的单链DNA上。
Bacillus subtilis RecO nucleates RecA onto SsbA-coated single-stranded DNA.
作者信息
Manfredi Candela, Carrasco Begoña, Ayora Silvia, Alonso Juan C
机构信息
Department of Microbial Biotechnology, Centro Nacional de Biotecnología, CSIC, C/Darwin 3, Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain.
出版信息
J Biol Chem. 2008 Sep 5;283(36):24837-47. doi: 10.1074/jbc.M802002200. Epub 2008 Jul 3.
Abstract
Subsaturating amounts of Bacillus subtilis SsbA, independently of the order of addition, partially inhibit the single-stranded DNA-dependent dATPase activity of RecA. This negative effect is fully overcome when a substoichiometric amount of RecO is added. SsbA added prior to RecA does not stimulate the dATP-dependent DNA strand exchange activity; however, added after RecA it enhances the extent of strand exchange. The addition of RecO stimulates RecA-mediated joint molecule formation, although it limits the accumulation of final recombination products. Thus we suggest that RecO has a dual activity: RecO acts as a RecA mediator enabling RecA to utilize SsbA-coated single-stranded DNA as a polymerization substrate and controls RecA-mediated DNA strand exchange by limiting its extent. We herein discuss the possible mechanisms of RecO involvement in the regulation of double strand break repair and genetic transformation.
摘要
枯草芽孢杆菌SsbA的亚饱和量，与添加顺序无关，部分抑制RecA的单链DNA依赖性dATPase活性。当添加亚化学计量的RecO时，这种负面影响会被完全克服。在RecA之前添加SsbA不会刺激dATP依赖性DNA链交换活性；然而，在RecA之后添加则会增强链交换的程度。添加RecO会刺激RecA介导的联合分子形成，尽管它会限制最终重组产物的积累。因此，我们认为RecO具有双重活性：RecO作为RecA的介质，使RecA能够将SsbA包被的单链DNA用作聚合底物，并通过限制其程度来控制RecA介导的DNA链交换。我们在此讨论RecO参与双链断裂修复和遗传转化调控的可能机制。
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