Liu Wenwei, Tang Qizhu, Jiang Hua, Ding Xiangwu, Liu Yongsheng, Zhu Rui, Tang Yongqian, Li Bin, Wei Min
Department of Cardiology, Xiangfan Central Hospital, Xiangfan, Hubei, China.
Angiology. 2009 Apr-May;60(2):180-5. doi: 10.1177/0003319708319939. Epub 2008 Jul 3.
Interleukin 18 (IL-18) is a pro-atherogenic cytokine associated with the occurrence of various cardiac complications. The IL-18 gene has a functional -137 G/C polymorphism (rs187238) in the promoter region. Using the ligase detection reaction-polymerase chain reaction, we genotyped a cohort of patients in Chinese Han population in Xiangfan region. Case patients of coronary artery disease and control patients were identified by coronary angiography. The plasma IL-18 concentrations were measured by ELISA. A significant increase of G allele or GG-genotype was observed in 241 case patients compared to 145 control individuals (frequency of G allele = 0.90 vs 0.83, p=0.004; frequency of GG-genotype = 0.81 vs 0.68, p = 0.005). In case patients, G allele carriers in multi-vessel disease patients had a higher occurrence rate when compared to single-vessel disease patients, but no significant difference was detected (frequency of G allele = 0.92 vs 0.88, p=0.107; frequency of GG-genotype = 0.84 vs 0.75, p = 0.089). IL-18 protein concentration of the -137GG genotype was much higher than concentration of the CG and CC genotype (case patients: 229.1+/-131.5 vs 122.7+/-73.6 pg/ml, P < 0.001; control patients: 65.9+/-31.6 vs 42.4+/-19.5 pg/ml, P < 0.001). To conclude, IL-18 promoter -137G/C polymorphism influences IL-18 levels and the occurrence of coronary artery disease, suggesting that IL-18 is causally involved in the development of atherosclerosis.
白细胞介素18（IL-18）是一种促动脉粥样硬化细胞因子，与多种心脏并发症的发生有关。IL-18基因在启动子区域存在功能性-137G/C多态性（rs187238）。我们采用连接酶检测反应-聚合酶链反应对襄樊地区中国汉族人群的一组患者进行基因分型。通过冠状动脉造影确定冠心病患者和对照患者。采用酶联免疫吸附测定法（ELISA）检测血浆IL-18浓度。与145名对照个体相比，241例患者中观察到G等位基因或GG基因型显著增加（G等位基因频率=0.90对0.83，p=0.004；GG基因型频率=0.81对0.68，p=0.005）。在患者中，多支血管病变患者的G等位基因携带者发生率高于单支血管病变患者，但差异无统计学意义（G等位基因频率=0.92对0.88，p=0.107；GG基因型频率=0.84对0.75，p=0.089）。-137GG基因型的IL-18蛋白浓度远高于CG和CC基因型（患者：229.1±131.5对122.7±73.6 pg/ml，P<0.001；对照患者：65.9±31.6对42.4±19.5 pg/ml，P<0.001）。总之，IL-18启动子-137G/C多态性影响IL-18水平和冠状动脉疾病的发生，提示IL-18与动脉粥样硬化的发生存在因果关系。
