Smith Julie L, Campbell Barbara J, Hanson Thomas E, Zhang Chuanlun L, Cary S Craig
University of Delaware, College of Marine Studies, Lewes, DE 19958, USA.
Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1598-602. doi: 10.1099/ijs.0.65435-0.
A thermophilic, strictly anaerobic, sulfur-reducing epsilonproteobacterium (strain AmH(T)) isolated from deep-sea hydrothermal vents is described. Cells were motile, Gram-negative rods. Growth was observed at 30-55 degrees C, pH 6.0-9.0 and 2-5 % (w/v) NaCl. Chemolithoautotrophic growth occurred with molecular hydrogen or formate as the electron donor and elemental sulfur as the electron acceptor, producing hydrogen sulfide. Heterotrophic and mixotrophic growth occurred with formate as a source of carbon. The dominant phospholipid fatty acids were C(18 : 1)omega7c (73.26 % of the total), C(16 : 1)omega7c (12.70 %) and C(16 : 0) (12.27 %). The genomic DNA G+C content was 33.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain AmH(T) within the family Nautiliaceae of the Epsilonproteobacteria. DNA-DNA hybridization experiments between strain AmH(T) and Nautilia lithotrophica DSM 13520(T) revealed a level of relatedness of 34.6 % between the two strains. Based on physiological and phylogenetic characteristics, strain AmH(T) is considered to represent a novel species of the genus Nautilia, for which the name Nautilia profundicola sp. nov. is proposed. The type strain is AmH(T) (=ATCC BAA-1463(T) =DSM 18972(T)).
本文描述了从深海热液喷口分离出的一株嗜热、严格厌氧、硫还原的ε-变形菌（菌株AmH(T)）。细胞呈运动性，为革兰氏阴性杆菌。在30 - 55℃、pH 6.0 - 9.0和2 - 5%（w/v）NaCl条件下可观察到生长。以分子氢或甲酸盐作为电子供体、元素硫作为电子受体时发生化能自养生长，产生硫化氢。以甲酸盐作为碳源时发生异养和混合营养生长。主要的磷脂脂肪酸为C(18 : 1)ω7c（占总量的73.26%）、C(16 : 1)ω7c（12.70%）和C(16 : 0)（12.27%）。基因组DNA的G + C含量为33.5 mol%。基于16S rRNA基因序列的系统发育分析将菌株AmH(T)置于ε-变形菌纲的Nautiliaceae科内。菌株AmH(T)与嗜岩石纳氏菌DSM 13520(T)之间的DNA - DNA杂交实验表明，两菌株间的相关性水平为34.6%。基于生理和系统发育特征，菌株AmH(T)被认为代表纳氏菌属的一个新物种，为此提出新名称深海纳氏菌（Nautilia profundicola sp. nov.）。模式菌株为AmH(T)（=ATCC BAA - 1463(T) =DSM 18972(T)）。
