与NoRC相关的RNA的结构对于将染色质重塑复合物NoRC靶向核仁至关重要。
The structure of NoRC-associated RNA is crucial for targeting the chromatin remodelling complex NoRC to the nucleolus.
作者信息
Mayer Christine, Neubert Melanie, Grummt Ingrid
机构信息
Division of Molecular Biology of the Cell II, German Cancer Research Center, DKFZ-ZMBH-Alliance, INF 581, D-69120 Heidelberg, Germany.
出版信息
EMBO Rep. 2008 Aug;9(8):774-80. doi: 10.1038/embor.2008.109. Epub 2008 Jul 4.
Abstract
Silencing of ribosomal RNA genes (rDNA) requires binding of the chromatin remodelling complex NoRC to RNA that is complementary to the rDNA promoter. NoRC-associated RNA (pRNA) folds into a conserved stem-loop structure that is required for nucleolar localization and rDNA silencing. Mutations that disrupt the stem-loop structure impair binding of TIP5, the large subunit of NoRC, to pRNA and abolish targeting of NoRC to nucleoli. Binding to pRNA results in a conformational change of TIP5, as shown by enhanced sensitivity of TIP5 towards trypsin digestion. Our results indicate an RNA-dependent mechanism that targets NoRC to chromatin and facilitates the interaction with co-repressors that promote heterochromatin formation and rDNA silencing.
摘要
核糖体RNA基因（rDNA）的沉默需要染色质重塑复合物NoRC与与rDNA启动子互补的RNA结合。NoRC相关RNA（pRNA）折叠成一种保守的茎环结构，这是核仁定位和rDNA沉默所必需的。破坏茎环结构的突变会损害NoRC的大亚基TIP5与pRNA的结合，并消除NoRC对核仁的靶向作用。与pRNA的结合导致TIP5的构象变化，如TIP5对胰蛋白酶消化的敏感性增强所示。我们的结果表明了一种RNA依赖性机制，该机制将NoRC靶向染色质，并促进与促进异染色质形成和rDNA沉默的共抑制因子的相互作用。
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