Tomkova J, Friedecky D, Vyskocilova P, Adam T
Department of Clinical Biochemistry, Laboratory for Inherited Metabolic Disorders, Medical Hospital, Olomouc, Czech Republic.
Nucleosides Nucleotides Nucleic Acids. 2008 Jun;27(6):656-60. doi: 10.1080/15257770802143897.
Inosine triphosphate pyrophosphohydrolase (ITPase) catalyzing the pyrophosphohydrolysis of inosine triphosphate, deoxyinosine triphosphate and xanthosine triphosphate is involved in the metabolism and tolerance of thiopurine drugs. ITPase activity plays an important role in the prediction of toxicity to thiopurine therapy. Activities in dry blood spots were compared with fresh erythrocytes. Samples were incubated with inosine triphosphate, then inosine monophosphate was determined by a capillary electrophoresis method. Calculated enzyme activities obtained from dry blood spots were in good accordance with activity in fresh erythrocytes.
