Nurnberger F, Lee T F, Jourdan M L, Wang L C
Department of Anatomy and Cytobiology, Justus Liebig University, Giessen, F.R.G.
Brain Res. 1991 Apr 26;547(1):115-21. doi: 10.1016/0006-8993(91)90581-f.
To identify the actual location of central endogenous opioid systems which may be involved in regulating the hibernation cycle, differences in the pattern of central methionine-enkephalin (Met-EK) immunoreactivity were compared between hibernating (body temperature, Tb = 7 degrees C) and non-hibernating (Tb = 37 degrees C) Columbian ground squirrels using the peroxidase-antiperoxidase technique. In non-hibernating animals, Met-EK-immunoreactive perikarya were observed in telencephalic (putamen, caudate nucleus, medial septum-diagonal band complex, amygdala) and diencephalic (periventricular hypothalamic nucleus, lateral hypothalamic area) regions, whereas immunoreactive fibers were found in the lateral septum, stria terminalis nucleus, various hypothalamic areas, arcuate nucleus, median eminence, thalamic intralaminar, periventricular nucleus and lateral habenular nucleus. Compared to the non-hibernating animal, a marked increase in the number of Met-EK-immunoreactive fibers was found in the lateral septal nucleus, the periventricular nucleus, the intralaminar thalamus and the paraventricular hypothalamus of hibernating ground squirrels. Since these changes in immunoreactivity were not observed in the artificially induced hypothermic ground squirrels (Tb = 7 degrees C), it is unlikely that the dissimilarity in immunoreactivity between animals from different hibernating phases is due to differences in their Tb. In combination with our previous studies, these results tend to suggest that hibernation may be brought about by an increase in endogenous opioid activity, especially in the lateral septal region.
为了确定可能参与调节冬眠周期的中枢内源性阿片系统的实际位置，采用过氧化物酶 - 抗过氧化物酶技术，比较了冬眠（体温，Tb = 7℃）和非冬眠（Tb = 37℃）的哥伦比亚地松鼠中枢甲硫氨酸脑啡肽（Met - EK）免疫反应性模式的差异。在非冬眠动物中，在端脑（壳核、尾状核、内侧隔 - 斜角带复合体、杏仁核）和间脑（室旁下丘脑核、外侧下丘脑区）区域观察到Met - EK免疫反应性神经元胞体，而在外侧隔、终纹核、各种下丘脑区域、弓状核、正中隆起、丘脑板内核、室旁核和外侧缰核中发现了免疫反应性纤维。与非冬眠动物相比，在冬眠地松鼠的外侧隔核、室旁核、丘脑板内核和室旁下丘脑中，发现Met - EK免疫反应性纤维的数量显著增加。由于在人工诱导低温的地松鼠（Tb = 7℃）中未观察到这些免疫反应性变化，不同冬眠阶段动物之间免疫反应性的差异不太可能是由于它们体温的差异。结合我们之前的研究，这些结果倾向于表明冬眠可能是由内源性阿片活性增加引起的，特别是在外侧隔区域。
