一种分离火鸡呼吸道腺病毒的技术。
A technique for isolating turkey respiratory adenoviruses.
作者信息
Simmons D G, Gray J G
出版信息
Avian Dis. 1976 Oct-Dec;20(4):669-75.
PMID:186007
Abstract
Several turkey respiratory adenoviruses were isolated in turkey embryonic liver cells from nasal turbinate filtrate collected from clinically ill turkey poults during postmortem examination. Suspect adenovirus inoculum had to remain in cell culture for 5 to 7 days, and 9 blind passages were required before a sample was declared negative. Once isolated, the turkey adenoviruses adapted rapidly to turkey kidney cells.
摘要
在尸检期间，从临床患病小火鸡的鼻甲滤液中收集样本，并在火鸡胚胎肝细胞中分离出几种火鸡呼吸道腺病毒。可疑腺病毒接种物必须在细胞培养中留存5至7天，且在样本被宣布为阴性之前需要进行9次盲传。一旦分离出来，火鸡腺病毒能迅速适应火鸡肾细胞。
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