CNRS-Laboratoire de Chimie Bactérienne, Université de Provence, marseille Cedex 9, France.
Biotechnol Bioeng. 1991 Jul;38(3):304-13. doi: 10.1002/bit.260380312.
A new osmotolerant mutant strain of Zymomonas mobilis was successfully used for ethanol production from beet molasses. Addition of magnesium sulfate to hydrolyzed molasses allowed repeated growth without the need of yeast extract addition. The kinetics and yields parameters of fermentation on media with different molasses concentrations were calculated. The anabolic parameters (specific growth rate, mu, and biomass yield, Y(X/S)) were inhibited at elevated molasses concentrations while the catabolic parameters (specific ethanol productivity, q(p), and ethanol yield, Y(p/s)) were not significantly affected. In addition to ethanol and substrate inhibition, osmotic pressure effects can explain the observed results.
成功地使用一种新的渗透耐受突变株运动发酵单胞菌从糖蜜中生产乙醇。向水解糖蜜中添加硫酸镁可以允许在无需添加酵母提取物的情况下进行重复生长。计算了在不同糖蜜浓度的培养基上发酵的动力学和产率参数。在高糖蜜浓度下，合成参数（比生长速率μ和生物质产率 Y(X/S)）受到抑制，而分解参数（比乙醇生产力 q(p)和乙醇产率 Y(p/s)）则没有受到显著影响。除了乙醇和基质抑制之外，渗透压效应也可以解释观察到的结果。
