Maruhashi F, Murakami S, Baba K
Hitachi Research Laboratory, Hitachi Ltd., Ibaraki, Japan.
Cytotechnology. 1994;15(1-3):281-9. doi: 10.1007/BF00762403.
In order to automate measurements of cell concentration and viability in a suspended animal cell culture, we have developed an in situ microscopic image analysis system with an effective cell recognition algorithm. With a small amount of sample, this system can measure the cell density rapidly and aseptically. In addition, it can measure a cell size histogram including cell debris small particle distribution. These small particles have been found to be related to the viability of the mouse-mouse hybridoma STK1 cell line. By using cell debris small particle density as an indicator of cell viability, the developed system provides non-destructive viability monitoring without trypan blue staining.
为了实现悬浮动物细胞培养中细胞浓度和活力测量的自动化，我们开发了一种具有有效细胞识别算法的原位显微图像分析系统。该系统只需少量样品，就能快速、无菌地测量细胞密度。此外，它还能测量包括细胞碎片和小颗粒分布在内的细胞大小直方图。已发现这些小颗粒与小鼠 - 小鼠杂交瘤STK1细胞系的活力有关。通过将细胞碎片小颗粒密度作为细胞活力指标，所开发的系统无需台盼蓝染色即可提供非破坏性的活力监测。
