Institute of Catalusis, Novosibirsk 630090, USSR.
Biotechnol Bioeng. 1992 Mar 5;39(5):522-8. doi: 10.1002/bit.260390507.
Immobilization of gas-utilizing microorganism strains (Mycobacteria, Rhodococcus, methane-utilizers) on inorganic supports based on alumina, silicates, and carbon was carried out to develop heterogeneous biocatalysts for the biotechnologic processes, including the process of propene epoxidation. Adsorption ability of these microorganisms, biocatalytic properties of resting and immobilized bacterial cells, and effect of immobilization techniques on biocatalysis were studied. An approach of double immobilization using inorganic materials (supports and gel) was proposed as simple, universal, and available method to immobilize bacterial cells, resulting in a higher retention (up to 100%) of cells' enzymatic activity and enhanced stability.
将利用气体的微生物菌株（分枝杆菌、红球菌、甲烷利用菌）固定在氧化铝、硅酸盐和碳为基础的无机载体上，以开发用于生物技术过程的非均相生物催化剂，包括丙烯环氧化过程。研究了这些微生物的吸附能力、休眠和固定化细菌细胞的生物催化特性，以及固定化技术对生物催化的影响。提出了一种使用无机材料（载体和凝胶）双重固定化的方法，作为一种简单、通用且可行的方法来固定化细菌细胞，从而使细胞的酶活性保留率（高达 100%）更高，稳定性增强。
