Laboratorio de Biotecnología Microbiana, Facultad de Farmacia, Universidad de Valparaíso, Casilla 5001, Valparaíso, Chile.
Bioresour Technol. 2010 Apr;101(7):2375-8. doi: 10.1016/j.biortech.2009.11.086.
The immobilization of Pseudomonas stutzeri using adsorption on different inorganic supports was studied in relation to the number of adsorbed cells, metabolic activity and biodesulfurization (BDS). The electrophoretic migration (EM) measurements and Tetrazolioum (TTC) method were used to evaluate adsorption and metabolic activity. Results indicate that maximal immobilization was obtained with an initial load of 14 x 10(8) cells mL(-1) for Al and Sep, whereas Ti requires 20 x 10(8) cells mL(-1). The highest interaction was observed in the P. stutzeri/Si and P. stutzeri/Sep biocatalysts. The IEP values and metabolic activities indicate that P. stutzeri change the surface of supports and maintains metabolic activity. A direct relation between BDS activity and the adsorption capacity of the bacterial cells was observed at the adsorption/desorption equilibrium level. The biomodification of inorganic supports by the adsorption process increases the bioavailability of sulphur substrates for bacterial cells, improving BDS activity.
采用吸附法将恶臭假单胞菌固定在不同的无机载体上，研究了吸附细胞数量、代谢活性与生物脱硫（BDS）之间的关系。采用电泳迁移（EM）测量和四唑（TTC）法评估吸附和代谢活性。结果表明，对于 Al 和 Sep，最大固定化程度在初始负载 14×10(8)个细胞/mL 时获得，而 Ti 需要 20×10(8)个细胞/mL。在 P. stutzeri/Si 和 P. stutzeri/Sep 生物催化剂中观察到最高的相互作用。等电点（IEP）值和代谢活性表明，P. stutzeri 改变了载体的表面并保持了代谢活性。在吸附/解吸平衡水平上，观察到 BDS 活性与细菌细胞吸附能力之间存在直接关系。吸附过程对无机载体的生物修饰增加了细菌细胞对硫底物的生物利用度，从而提高了 BDS 活性。
