Department of Chemical Engineering, University of Colorado, Boulder, Colorado 80309-0424, USA.
Biotechnol Bioeng. 1992 Mar 15;39(6):635-46. doi: 10.1002/bit.260390608.
A new generalized mathematical model for recombinant bacteria which includes inducer effects on cell growth and foreign protein production is developed. The model equation set was applied to a host-vector system, Escherichi coli D1210 and plasmid pSD8. Batch experiments were designed and performed in shake flasks to verify the model. A parameter estimation method was developed and proven to be efficient. Although simple, the model can effectively describe the dynamics of the production of foreign protein in recombinant bacteria and can be used for optimization and control studies to maximize foreign protein production.
建立了一个包含诱导剂对细胞生长和外源蛋白生产影响的重组菌通用的数学模型。模型方程组应用于宿主-载体系统大肠杆菌 D1210 和质粒 pSD8。通过摇瓶分批实验对模型进行了验证。提出了一种参数估计方法，并证明其是有效的。尽管模型比较简单，但它能有效地描述重组菌中外源蛋白的生产动力学，可以用于优化和控制研究以最大限度地提高外源蛋白的产量。
