Donovan R S, Robinson C W, Glick B R
Department of Chemical Engineering, University of Waterloo, Ontario, Canada.
J Ind Microbiol. 1996 Mar;16(3):145-54. doi: 10.1007/BF01569997.
This review examines factors which influence the expression of foreign proteins in Escherichia coli under the transcriptional control of the lac and tac promoters, and discusses conditions for maximizing the production of a foreign protein using this system. Specifically, the influence of IPTG (isopropyl-beta-D-thiogalactoside) concentration, temperature, composition of the growth medium, the point in the growth curve at which cells are induced with either IPTG or lactose, and the duration of the induction phase are discussed.
本综述研究了在乳糖启动子和tac启动子的转录控制下，影响外源蛋白在大肠杆菌中表达的因素，并讨论了使用该系统最大化外源蛋白产量的条件。具体而言，讨论了异丙基-β-D-硫代半乳糖苷（IPTG）浓度、温度、生长培养基组成、用IPTG或乳糖诱导细胞时在生长曲线中的时间点以及诱导阶段的持续时间的影响。
