Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1501, USA.
Biotechnol Bioeng. 1992 Dec 5;40(10):1210-7. doi: 10.1002/bit.260401011.
The optimum experimental conditions (sample size and mobile phase composition) are calculated for maximum production rate of either one of two enantiomers contained in feeds of different compositions (1/1, 1/10, and 10/1). The products are obtained at 99% purity. The calculations use the equilibrium-dispersive model of chromatography and the equilibrium isotherms determined experimentally from the rear, diffuse boundary of overloaded elution profiles. The production rate measured experimentally under the optimum conditions calculated agree with 4% of the calculated values. There is an optimum value for the retention factor which is higher than predicted by a model assuming constant separation factor, because both separation factor and retention decrease with increasing organic solvent concentration in the mobile phase.
计算了最优实验条件（样品量和流动相组成），以最大限度地提高不同组成（1/1、1/10 和 10/1）进料中两种对映异构体之一的生产速率。产物的纯度达到 99%。计算使用色谱的平衡弥散模型和从过载洗脱曲线的后扩散边界实验确定的平衡等温线。在计算出的最佳条件下进行的实验生产速率与计算值的 4%相符。保留因子存在最佳值，其高于假设分离因子恒定的模型的预测值，因为分离因子和保留因子都随流动相中有机溶剂浓度的增加而降低。
