Felinger A, Guiochon G
Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1501, USA.
Biotechnol Bioeng. 1993 Jan 5;41(1):134-47. doi: 10.1002/bit.260410118.
The results of a study of the optimization of the experimental conditions for maximum production rate in overloaded elution and displacement chromatography are discussed. This study is based on the use of the equilibrium-dispersive model of chromatography and the competitive Langmuir isotherms to calculate individual band profiles in the elution and displacement modes, and of a simplex algorithm to optimize the production rate. The operating parameters (sample size, mobile phase velocity, and the displacer concentration in the displacement model) and the column design parameters (column length and average particle diameter) are optimized simultaneously. Binary mixtures having relative concentrations 3:1 and 1:3, and separation factors of 1.2 to 1.8 are investigated. One of our major results is that, in both modes of chromatography, the maximum production rate is achieved at very low values of the retention factors, k', much lower than those used in current practice. In all cases, unless k' exceeds greatly that optimum value, the production rate is higher in overloaded elution than in displacement chromatography. This is particularly true for the extraction of a minor component, which is eluted second.
本文讨论了一项关于优化过载洗脱色谱和顶替色谱实验条件以实现最大生产率的研究结果。该研究基于色谱的平衡扩散模型和竞争朗缪尔等温线来计算洗脱模式和顶替模式下的单个谱带轮廓，并采用单纯形算法来优化生产率。同时对操作参数（进样量、流动相流速以及顶替模型中的顶替剂浓度）和色谱柱设计参数（柱长和平均粒径）进行了优化。研究了相对浓度为3:1和1:3且分离因子为1.2至1.8的二元混合物。我们的一个主要结果是，在两种色谱模式下，最大生产率均在非常低的保留因子k'值下实现，远低于当前实际应用中的值。在所有情况下，除非k'大大超过该最佳值，否则过载洗脱的生产率高于顶替色谱。对于第二个洗脱的次要组分的萃取尤其如此。
