Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, 30043 ROC.
Biotechnol Bioeng. 1992 Dec 20;40(11):1300-8. doi: 10.1002/bit.260401103.
The mechanical stability of liquid-core alginate-poly-L-lysine (PLL) capsules used for encapsulation of hybridoma cells can be greatly enhanced by the inclusion of poly(ethylenimine) (PEI) in the hardening solution containing calcium chloride. The PEI can also reinforce the PLL-coated carboxymethyl-celluose liquid-core capsules. The cultivation of murein hybridoma CT04 in these two capsules was carried out. Cell concentrations higher than 10(8) cells/mL per capsule were obtained with ca. 80% of the specific antibody productivity as the freely suspended cells. These capsules could withstand severe agitation and aeration in an air-lift reactor over a period of 3 weeks with minimal damage.
通过在包含氯化钙的硬化溶液中加入聚（亚乙基亚胺）（PEI），可以大大提高用于包封杂交瘤细胞的液体芯海藻酸钠-聚-L-赖氨酸（PLL）胶囊的机械稳定性。PEI 还可以增强涂有 PLL 的羧甲基纤维素液体芯胶囊。在这两种胶囊中培养了细胞壁杂交瘤 CT04。每个胶囊中的细胞浓度高于 10（8）个细胞/mL，并且具有与自由悬浮细胞相当的约 80%的特异性抗体生产率。这些胶囊可以在空气升力反应器中经受剧烈搅拌和通气 3 周而几乎没有损坏。
