Center for Interfacial Microbial Process Engineering, Montana State University, Bozeman, Montana 59717, USA.
Biotechnol Bioeng. 1992 Sep;40(6):725-34. doi: 10.1002/bit.260400612.
The effects of sulfate and nitrogen concentrations of the rate and stoichiometry of microbial sulfate reduction were investigated for Desulfovibrio desulfuricans grown on lactate and sulfate in a chemostat at pH 7.0. Maximum specific growth rates (micro(max)), half-saturation coefficients (K(sul)), and cell yield (Y(c/Lac)) of 0.344 +/- 0.007 and 0.352 +/- 0.003 h (-1), 1.8 +/- 0.3 and 1.0 +/- 0.2 mg/L, and 0.020 +/- 0.003 and 0.017 +/- 0.003 g cell/g lactate, respectively, were obtained under sulfate-limiting conditions at 35 degrees C and 43 degrees C. Maintenance energy requirements for D. desulfuricans were significant under sulfate-limiting conditions. The extent of extracellular polymeric substance (EPS) produced was related to the carbon: nitrogen ratio in the medium. EPS production rate increased with decreased nitrogen loading rate. Nitrogen starvation also resulted in decreased cell size of D. desulfuricans. The limiting C : N ratio (w/w) for D. desulfuricans was in the range of 45 : 1 to 120 : 1. Effects of sulfide on microbial sulfate reduction, cell size, and biomass production were also investigated at pH 7.0. Fifty percent inhibition of lactate utilization occurred at a total sulfide concentration of approximately 500 mg/L. The cell size of D. desulfuricans decreased with increasing total sulfide concentration. Sulfide inhibition of D. desulfuricans was observed to be a reversible process.
在 pH 值为 7.0 的恒化器中，利用硫酸盐和乳酸盐培养脱硫弧菌，研究了硫酸盐和氮浓度对其硫酸盐还原速率和化学计量的影响。在 35°C 和 43°C 下，硫酸盐限制条件下的最大比生长速率（μ(max)）、半饱和系数（K(sul)）和细胞产率（Y(c/Lac)）分别为 0.344 ± 0.007 和 0.352 ± 0.003 h(-1)、1.8 ± 0.3 和 1.0 ± 0.2 mg/L，以及 0.020 ± 0.003 和 0.017 ± 0.003 g 细胞/g 乳酸盐。在硫酸盐限制条件下，脱硫弧菌的维持能量需求非常显著。胞外聚合物（EPS）的产生程度与培养基中的碳：氮比有关。随着氮负荷率的降低，EPS 产生速率增加。氮饥饿还导致脱硫弧菌细胞尺寸减小。脱硫弧菌的限制 C:N 比（w/w）在 45:1 至 120:1 之间。在 pH 值为 7.0 时，还研究了硫化物对微生物硫酸盐还原、细胞大小和生物量产生的影响。乳酸盐利用率的 50%抑制发生在总硫化物浓度约为 500mg/L 时。脱硫弧菌的细胞尺寸随总硫化物浓度的增加而减小。观察到硫化物对脱硫弧菌的抑制是一个可逆的过程。
