Okabe S, Characklis W G
Center for Interfacial Microbial Process Engineering, Montana State University, Bozeman, Montana 59717, USA.
Biotechnol Bioeng. 1992 Apr 25;39(10):1031-42. doi: 10.1002/bit.260391007.
The effects of temperature and phosphorous concentration on the rate and the extent of microbial sulfate reduction with lactate as carbon and energy source were investigated for Desulfovibrio desulfuricans. The continuous culture experiments (chemostat) were conducted at pH 7.0 from 12 to 48 degrees C. The maximum specific growth rate (micro(max)) was relatively constant in the range 25 degrees C-43 degrees C and dramatically decreased outside this temperature range. The half-saturation coefficient was minimum at 25 degrees C. Cell yield was highest in the optimum temperature range (35 degrees C-43 degrees C) for growth. Maintenance energy requirements for D. desulfuricans were not significant. Two moles of lactate is consumed for every mole of sulfate reduced, and this stoichiometric ratio is not temperature dependent. Steady state rate and stoichiometric coefficients accurately predicted transient behavior during temperature shifts. The extent of extracellular polymeric substance (EPS) is related to the concentration of phosphorous in the medium. EPS production rate increased with decreased phosphorous loading rate. Failure to discriminate between cell and EPS formation by D. desulfuricans leads to significant overestimates of the cell yield. The limiting C:P ratio for D. desulfuricans was in the range of 400:1 to 800:1.
以乳酸盐作为碳源和能源，研究了温度和磷浓度对脱硫脱硫弧菌微生物硫酸盐还原速率和程度的影响。连续培养实验（恒化器）在pH 7.0、12至48摄氏度的条件下进行。最大比生长速率（μmax）在25摄氏度至43摄氏度范围内相对恒定，在此温度范围之外则显著下降。半饱和系数在25摄氏度时最小。细胞产量在生长的最佳温度范围（35摄氏度至43摄氏度）内最高。脱硫脱硫弧菌的维持能量需求不显著。每还原一摩尔硫酸盐消耗两摩尔乳酸盐，且该化学计量比与温度无关。稳态速率和化学计量系数准确预测了温度变化期间的瞬态行为。胞外聚合物（EPS）的程度与培养基中磷的浓度有关。EPS产生速率随磷负载率的降低而增加。未能区分脱硫脱硫弧菌的细胞形成和EPS形成会导致对细胞产量的显著高估。脱硫脱硫弧菌的限制碳磷比在400:1至800:1的范围内。
