Institute of Biomedical Engineering, Institute of Biochemistry, National Yang Ming Medical College, Taipei, Taiwan, Republic of China.
Biotechnol Bioeng. 1992 Nov;40(9):1115-8. doi: 10.1002/bit.260400916.
Poly(vinylamine) was synthesized and used to replace poly-L-lysine in forming microcapsule with alginate. Test results indicated that capsules with good mechanical strength and permeability could be obtained under the controlled treatment conditions of poly(vinylamine) and alginate. Application of the current microcapsular system to cell culture was demonstrated by the usage of erythropoietin- (EPO-) producing IW32 mouse erythroleukemia cells. The encapsulated IW32 cells grew to a density of 8 x 10(7) cells/mL, two times that found in the corresponding poly-L-lysine/alginate capsules. The EPO accumulation inside the microcapsule with the current encapsulation system was also higher. A concentration of 7.3 U/mL was attained as compared to 4.3 U/mL in the poly-L-lysine/alginate microcapsule.
聚（乙烯胺）被合成并用于替代聚-L-赖氨酸与藻酸盐形成微胶囊。实验结果表明，在控制聚（乙烯胺）和藻酸盐处理条件下，可以得到机械强度和渗透性良好的胶囊。当前的微囊系统通过使用产生促红细胞生成素（EPO）的 IW32 小鼠红白血病细胞进行细胞培养应用。包封的 IW32 细胞的密度达到 8 x 10(7)细胞/毫升，是相应的聚-L-赖氨酸/藻酸盐胶囊中的两倍。当前封装系统中的微胶囊内 EPO 的积累也更高。与聚-L-赖氨酸/藻酸盐微胶囊中的 4.3 U/mL 相比，达到了 7.3 U/mL 的浓度。
