Oreopoulos John, Yip Christopher M
Institute of Biomaterials and Biomedical Engineering, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College St, Toronto, Ont., Canada M5S 3E1.
Methods. 2008 Sep;46(1):2-10. doi: 10.1016/j.ymeth.2008.05.011. Epub 2008 Jul 2.
Combining scanning probe and optical microscopy represents a powerful approach for investigating structure-function relationships and dynamics of biomolecules and biomolecular assemblies, often in situ and in real-time. This platform technology allows us to obtain three-dimensional images of individual molecules with nanometer resolution, while simultaneously characterizing their structure and interactions though complementary techniques such as optical microscopy and spectroscopy. We describe herein the practical strategies for the coupling of scanning probe and total internal reflection fluorescence microscopy along with challenges and the potential applications of such platforms, with a particular focus on their application to the study of biomolecular interactions at membrane surfaces.
结合扫描探针显微镜和光学显微镜是研究生物分子及生物分子组装体的结构-功能关系与动力学的有力方法，通常可实现原位和实时研究。这种平台技术使我们能够获得具有纳米分辨率的单个分子的三维图像，同时通过光学显微镜和光谱学等互补技术表征其结构和相互作用。本文描述了扫描探针显微镜与全内反射荧光显微镜联用的实际策略，以及此类平台面临的挑战和潜在应用，特别关注其在研究膜表面生物分子相互作用方面的应用。
