Institute of Biomaterials and Biomedical Engineering, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Ontario, Canada.
Biophys J. 2010 Mar 3;98(5):815-23. doi: 10.1016/j.bpj.2009.12.4327.
Direct visualization of the mechanism(s) by which peptides induce localized changes to the structure of membranes has high potential for enabling understanding of the structure-function relationship in antimicrobial and cell-penetrating peptides. We have applied a combined imaging strategy to track the interaction of a model antimicrobial peptide, PFWRIRIRR-amide, with bacterial membrane-mimetic supported phospholipid bilayers comprised of POPE/TOCL. Our in situ studies revealed rapid reorganization of the POPE/TOCL membrane into localized TOCL-rich domains with a concomitant change in the organization of the membranes themselves, as reflected by changes in fluorescent-membrane-probe order parameter, upon introduction of the peptide.
直接观察肽诱导膜结构局部变化的机制，对于理解抗菌肽和细胞穿透肽的结构-功能关系具有很高的潜力。我们应用了一种组合成像策略来跟踪模型抗菌肽 PFWRIRIRR-amide 与由 POPE/TOCL 组成的细菌膜模拟支持磷脂双层的相互作用。我们的原位研究表明，POPE/TOCL 膜会迅速重组为局部富含 TOCL 的区域，同时膜本身的组织也会发生变化，这反映在荧光膜探针有序参数的变化上，在引入肽后会发生这种变化。
