Nho Young-Kyun, Ha Eunyoung, Yu Kyung-Im, Chung Joo-Ho, Wook Nam-Chang, Chung In-Sung, Lee Mi-Young, Shin Dong-Hoon
Department of Preventive Medicine, School of Medicine, and Institute for Medical Genetics, Keimyung University, 194 Dong San-Dong, Jung-Gu, Daegu 700-712, Republic of Korea.
Clin Chim Acta. 2008 Oct;396(1-2):14-7. doi: 10.1016/j.cca.2008.06.012. Epub 2008 Jun 15.
Obesity leads to serious medical complications and impairment of quality of life. We investigated whether inter-individual variability in the risk of obesity was associated with a crucial fibrillar collagen-degrading enzyme matrix metalloproteinase (MMP)-1 polymorphisms (MMP1-1607 and MMP1-519).
A population-based cohort study consisting of 530 subjects was performed. Body mass index (BMI), systolic and diastolic blood pressures, fasting blood sugar (FBS), HbA1c, total cholesterol, triglyceride, and high density lipoprotein (HDL)-cholesterol were measured. Study subjects divided into 2 groups, one with BMI<25.0 and the other with BMI>or=25.0, were genotyped for MMP1-1607 and MMP1-519 polymorphisms by pyrosequencing analysis.
Analyses of genotype distributions and allele frequencies revealed that both MMP1-1607 and MMP1-519 polymorphisms were associated with BMI (P=0.041 and 0.043, respectively) in individuals with age>or=50 years. We also observed significantly lower BMI and triglyceride in -519 AA individuals with age>or=50 years than in -519 G allele carriers with age>or=50 years. Logistic regression analysis revealed that the odds ratio (OR) for increase in BMI associated with the G vs. A allele in individuals with age>or=50 was 2.02 [95% confidence interval (CI), 1.13-3.60, P=0.01], which strongly implicates protective role of MMP1-519 A allele against increase in BMI.
The frequencies of MMP1-1607 G allele and MMP1-519 A allele are significantly higher in subjects with BMI<25.0 and age>or=50 years, suggesting protective roles of MMP1-1607 G allele and MMP1-519 A allele against increase in BMI in Korean population.
肥胖会导致严重的医学并发症并损害生活质量。我们研究了肥胖风险的个体间差异是否与一种关键的纤维状胶原降解酶基质金属蛋白酶（MMP）-1多态性（MMP1-1607和MMP1-519）相关。
进行了一项基于人群的队列研究，共纳入530名受试者。测量了体重指数（BMI）、收缩压和舒张压、空腹血糖（FBS）、糖化血红蛋白（HbA1c）、总胆固醇、甘油三酯和高密度脂蛋白（HDL）胆固醇。将研究对象分为两组，一组BMI<25.0，另一组BMI≥25.0，通过焦磷酸测序分析对MMP1-1607和MMP1-519多态性进行基因分型。
基因型分布和等位基因频率分析显示，在年龄≥50岁的个体中，MMP1-1607和MMP1-519多态性均与BMI相关（分别为P=0.041和0.043）。我们还观察到，年龄≥50岁的-519 AA个体的BMI和甘油三酯显著低于年龄≥50岁的-519 G等位基因携带者。逻辑回归分析显示，年龄≥50岁的个体中，与G等位基因相比，A等位基因导致BMI增加的优势比（OR）为2.02 [95%置信区间（CI），1.13-3.60，P=0.01]，这强烈表明MMP1-519 A等位基因对BMI增加具有保护作用。
在BMI<25.0且年龄≥50岁的受试者中，MMP1-1607 G等位基因和MMP1-519 A等位基因的频率显著更高，这表明在韩国人群中，MMP1-1607 G等位基因和MMP1-519 A等位基因对BMI增加具有保护作用。
