卵泡液诱导兔顶体反应活性的性质。

Nature of the rabbit acrosome reaction-inducing activity of follicular fluid.

作者信息

Oliphant G, Cabot C L, Singhas C A

出版信息

J Reprod Fertil. 1977 Jul;50(2):245-50. doi: 10.1530/jrf.0.0500245.

DOI:10.1530/jrf.0.0500245

Abstract

Follicular fluid samples from rabbits, cats, pigs, women and cows had acrosome reaction-inducing activity (ARIA) on rabbit spermatozoa as determined by differential staining after incubation with these fluids. Activity was retained after dialysis and at least 50% was found to be labile when heated to 56 degrees C for as little as 20 min. The induction of the acrosome reaction by bovine follicular fluid showed a dependence on the concentration of follicular fluid and spermatozoa and on calcium ions, and had a pH optimum of approximately 8. Enzyme treatments showed that proteases destroyed the ARIA and this activity was completely blocked by treatment of bovine follicular fluid with goat anti-bovine antiserum. Electron microscope observations indicated the similarity of the reactions observed to that occurring in vivo. It is concluded that the rabbit acrosome reaction inducing-activity of bovine follicular fluid is a serum component(s), probably a protein(s) of high molecular weight.

摘要

用兔、猫、猪、人及牛的卵泡液与兔精子孵育后，通过鉴别染色发现，这些动物的卵泡液对兔精子均具有顶体反应诱导活性（ARIA）。经透析后该活性依然存在，并且当在56℃加热仅20分钟时，至少50%的活性会丧失。牛卵泡液诱导顶体反应表现出对卵泡液和精子浓度以及钙离子的依赖性，最适pH约为8。酶处理表明蛋白酶可破坏ARIA，用山羊抗牛抗血清处理牛卵泡液可完全阻断该活性。电子显微镜观察表明，所观察到的反应与体内发生的反应相似。得出的结论是，牛卵泡液诱导兔顶体反应的活性是一种血清成分，可能是一种高分子量蛋白质。