Yin Huaqun, Cao Linhui, Xie Ming, Chen Qijiong, Qiu Guanzhou, Zhou Jizhong, Wu Liyou, Wang Dianzuo, Liu Xueduan
School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan, China.
Syst Appl Microbiol. 2008 Sep;31(4):302-11. doi: 10.1016/j.syapm.2008.05.003. Epub 2008 Jul 7.
The diversity of bacterial communities at three sites impacted by acid mine drainage (AMD) from the Yinshan Mine in China was studied using comparative sequence analysis of two molecular markers, the 16S rRNA and gyrB genes. The phylogenetic analyses retrieved sequences from six classes of bacteria, Nitrospira, Alphaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Acidobacteria, and Actinobacteria, as well as sequences related to the plastid of the cyanobacterium Cyanidium acidocaldarium and also some unknown bacteria. The results of phylogenetic analyses based on gyrB and 16S rRNA were compared. This confirmed that gyrB gene analysis may be a useful tool, in addition to the comparative sequence analysis of the 16S rRNA gene, for the analysis of microbial community compositions. Moreover, the Mantel test showed that the geochemical characteristics, especially the pH value and the concentration of iron, strongly influenced the composition of the microbial communities.
利用16S rRNA和gyrB基因这两种分子标记的比较序列分析方法，研究了中国银山矿酸性矿山废水（AMD）影响的三个地点细菌群落的多样性。系统发育分析从六类细菌中检索到序列，包括硝化螺旋菌、α-变形菌纲、γ-变形菌纲、δ-变形菌纲、酸杆菌纲和放线菌纲，以及与嗜酸热蓝藻质体相关的序列和一些未知细菌。比较了基于gyrB和16S rRNA的系统发育分析结果。这证实了除16S rRNA基因的比较序列分析外，gyrB基因分析可能是分析微生物群落组成的有用工具。此外，Mantel检验表明，地球化学特征，尤其是pH值和铁浓度，强烈影响微生物群落的组成。
