Wang Hui, Li Wen-Sheng, Shi De-Jin, Ye Zhuo-Peng, Tai Feng, He Hai-Yong, Liang Chao-Feng, Gong Jin, Guo Ying
Department of Neurosurgery, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China.
J Neurooncol. 2008 Nov;90(2):151-6. doi: 10.1007/s11060-008-9647-9. Epub 2008 Jul 5.
To explore the correlation of matrix metalloproteinase-1 (MMP(1)) and tissue inhibitor of metalloproteinase-1 (TIMP(1)) with pituitary adenoma fibrosis.
Thirty-eight pituitary adenoma specimens were divided into fibrous group (6 patients) and non-fibrous group (32 patients). MMP(1) and TIMP(1) expression was detected by RT-PCR and immunohistochemistry. The collagen content was determined by Sirius scarlet staining.
In fibrous and non-fibrous group: (1) the collagen content was 20.95 +/- 8.42% and 7.98 +/- 5.18% respectively, and there was a statistical significance (P < 0.01). (2) The expression of MMP(1)mRNA was 0.47 +/- 0.40 and 0.59 +/- 0.54 respectively and its protein expression was 0.12 +/- 0.09 and 0.13 +/- 0.09 respectively, and there was no statistical significance (P > 0.05). Their expression was not related to collagen content (P > 0.05). (3) The expression of TIMP(1)mRNA was 1.61 +/- 1.09 and 0.79 +/- 0.59 respectively and its protein expression was 0.58 +/- 0.11 and 0.32 +/- 0.18 respectively, and there was a statistical significance (P < 0.01). Their expression was related to collagen content (P < 0.01).
The pathological features of pituitary adenoma fibrosis are excessive collagen deposition. High expression of TIMP(1) may be an important cause.
探讨基质金属蛋白酶-1（MMP-1）和金属蛋白酶组织抑制剂-1（TIMP-1）与垂体腺瘤纤维化的相关性。
将38例垂体腺瘤标本分为纤维型组（6例患者）和非纤维型组（32例患者）。采用逆转录聚合酶链反应（RT-PCR）和免疫组织化学法检测MMP-1和TIMP-1的表达。用天狼星红染色法测定胶原含量。
在纤维型组和非纤维型组中：（1）胶原含量分别为20.95±8.42%和7.98±5.18%，差异有统计学意义（P<0.01）。（2）MMP-1mRNA表达分别为0.47±0.40和0.59±0.54，其蛋白表达分别为0.12±0.09和0.13±0.09，差异无统计学意义（P>0.05）。其表达与胶原含量无关（P>0.05）。（3）TIMP-1mRNA表达分别为1.61±1.09和0.79±0.59，其蛋白表达分别为0.58±0.11和0.32±0.18，差异有统计学意义（P<0.01）。其表达与胶原含量有关（P<0.01）。
垂体腺瘤纤维化的病理特征是胶原过度沉积。TIMP-1的高表达可能是一个重要原因。
