水通道蛋白1、3和4、钠钾氯协同转运蛋白1和钠钾氯协同转运蛋白2在人内淋巴囊中的表达。
Expression of aquaporin1, 3, and 4, NKCC1, and NKCC2 in the human endolymphatic sac.
作者信息
Kakigi Akinobu, Nishimura Masahiko, Takeda Taizo, Taguchi Daizo, Nishioka Rie
机构信息
Department of Otolaryngology, Kochi Medical School, Nankoku, Japan.
出版信息
Auris Nasus Larynx. 2009 Apr;36(2):135-9. doi: 10.1016/j.anl.2008.04.012. Epub 2008 Jul 7.
OBJECTIVE
To locate aquaporin (AQP) 1, 3, and 4, Na-K-2Cl cotransporter (NKCC) 1 and 2 in the human endolymphatic sac (ES).
METHODS
A sample of human ES was harvested during the removal of vestibular schwannoma via the translabyrinthine approach. The sample was immediately fixed in 4% paraformaldehyde and embedded in OCT compound. Immunohistochemistry was performed with AQP1, 3, and 4, NKCC1, and NKCC2 polyclonal antibodies.
RESULTS
AQP1, AQP3, and NKCC2 were strongly expressed in the epithelial layer of the ES. AQP4 and NKCC1 were weakly expressed in the epithelial layer of the ES.
CONCLUSIONS
As it is impossible to perform quantitative analysis based on the fluorescence intensity of each immunoreactivity, we have presented the existence of AQP1, 3, and 4, NKCC1, and NKCC2 in the ES. The expression of NKCC1 and 2 indicated that the ES may have both secretory and adsorptive functions to maintain the homeostasis of endolymph.
目的
在人内淋巴囊（ES）中定位水通道蛋白（AQP）1、3和4、钠-钾-2氯协同转运蛋白（NKCC）1和2。
方法
通过经迷路入路切除前庭神经鞘瘤时获取人ES样本。样本立即固定于4%多聚甲醛中，包埋于OCT复合物中。使用AQP1、3和4、NKCC1和NKCC2多克隆抗体进行免疫组织化学。
结果
AQP1、AQP3和NKCC2在ES的上皮层中强烈表达。AQP4和NKCC1在ES的上皮层中弱表达。
结论
由于无法基于每种免疫反应性的荧光强度进行定量分析，我们展示了ES中AQP1、3和4、NKCC1和NKCC2的存在。NKCC1和2的表达表明ES可能具有分泌和吸附功能以维持内淋巴的稳态。
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