Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Pathology, Peking University Cancer Hospital & Institute, Beijing, China.
Anal Cell Pathol (Amst). 2021 Oct 26;2021:7866471. doi: 10.1155/2021/7866471. eCollection 2021.
Aquaporin 3 (AQP3) is the membrane channel of water and involved in fluid homeostasis. The aim of this study was to reveal the expression and significance of AQP3 in cutaneous lesions. We analyzed AQP3 mRNA levels using RT-PCR in 311 cutaneous lesions and confirmed AQP3 expression in these lesions by immunohistochemistry. AQP3 mRNA was detected in normal epidermis, seborrheic keratosis, solar keratosis, Bowen's disease, squamous cell carcinoma, eccrine poroma, apocrine carcinoma, and sebaceoma; however, AQP3 mRNA was absent in basal cell carcinoma, nevocellular nevus, or malignant melanoma. By immunohistochemistry, diffuse AQP3 expression was seen in all keratotic lesions including seborrheic keratosis, verruca vulgaris, molluscum contagiosum, solar keratosis, Bowen's disease, and squamous cell carcinoma. Diffuse AQP3 expression was also present in all extramammary Paget's disease. No AQP3 staining was obtained in basal cell carcinoma. Positive AQP3 staining was seen in sweat gland tumors including hidradenoma, eccrine poroma, and apocrine carcinoma. Among sebaceous tumors, AQP3 expressed diffusely in all sebaceous hyperplasia and sebaceous adenoma, but not in sebaceous carcinomas. Only focal AQP3 staining was seen in nevocellular nevus and no AQP3 staining in melanoma. Our findings indicate the function of AQP3 maintained in most skin tumors. AQP3 may be used for differential diagnosis in skin tumors.
水通道蛋白 3（AQP3）是水的膜通道，参与体液平衡。本研究旨在揭示 AQP3 在皮肤病变中的表达和意义。我们通过 RT-PCR 分析了 311 个皮肤病变中的 AQP3 mRNA 水平，并通过免疫组织化学证实了这些病变中 AQP3 的表达。AQP3 mRNA 在正常表皮、脂溢性角化病、光化性角化病、鲍恩病、鳞状细胞癌、小汗腺汗孔瘤、大汗腺癌和皮脂瘤中均有检测到；然而，在基底细胞癌、色素痣或恶性黑色素瘤中则未检测到 AQP3 mRNA。通过免疫组织化学，所有角化病变包括脂溢性角化病、寻常疣、传染性软疣、光化性角化病、鲍恩病和鳞状细胞癌中均可见弥漫性 AQP3 表达。所有乳房外 Paget 病也存在弥漫性 AQP3 表达。基底细胞癌中未获得 AQP3 染色。在汗腺肿瘤包括汗腺瘤、小汗腺汗孔瘤和大汗腺癌中可见 AQP3 阳性染色。在皮脂腺肿瘤中，AQP3 在所有皮脂腺增生和皮脂腺腺瘤中弥漫表达，但在皮脂腺癌中则不表达。色素痣中仅见局灶性 AQP3 染色，而黑色素瘤中则未见 AQP3 染色。我们的研究结果表明 AQP3 在大多数皮肤肿瘤中保持功能。AQP3 可用于皮肤肿瘤的鉴别诊断。
